Advanced Molecular Methods in Biotechnology
| Code | School | Level | Credits | Semesters |
| BIOS4138 | Biosciences | 4 | 20 | Spring Malaysia |
- Code
- BIOS4138
- School
- Biosciences
- Level
- 4
- Credits
- 20
- Semesters
- Spring Malaysia
Summary
The ability to manipulate genetic information is central to biotechnology. This module provides in-depth understanding of manipulating cells and biological processes together with the application of this knowledge to genetic engineering. Practical methods involve isolating, characterising and functionally analysing genes. We are currently in an era of ‘’Omics’’. Over the past few years major developments have been made regarding the study of genomes. With the advent of next generation and third generation sequencing, whole genome sequences of many species are now available. Such information is revealing a high degree of similarity and conservation between different species and organisms, which in turn is revolutionising the way in which gene function analysis is carried out. An extensive range of post-genomic technologies have been established based on this information and these are revolutionising the analysis that is possible. Case studies will be presented detailing how different approaches have been used to study genomes and how such developments are influencing the way genetic analysis and biotechnological improvement can be made. Particular emphasis will be paid to the importance of bioinformatics in the study of genomes and their expression. Hands-on experience of these approaches will be provided via problem-based lab and computer training sessions. The methods explained by means of lectures, tutorials and practicals are applicable to plant, microbial and animal cells.
Target Students
Biosciences students. There are limited number of places on this module. Students are reminded that enrolments which are not agreed by the Offering School in advance may be cancelled without notice.A suitable range of molecular biology or genetic/bioinformatic modules in previous semesters. Available to JYA/Erasmus students.
Classes
- One 4-hour practicum each week for 3 weeks
- Two 2-hour lectures each week for 4 weeks
- One 2-hour lecture each week for 4 weeks
- One 3-hour laboratory each week for 5 weeks
27 hours Lectures 8 hours Practicals 13 hours Tutorial
Assessment
- 50% Coursework: Practical reports
- 50% Exam 1 (2-hour): 2 hour exam
Assessed by end of autumn semester
Educational Aims
To introduce the student to modern molecular methods in biotechnology. Particular focus will be on transgenis methods and the use and application of bioinformatics in understanding the functional roles of genes, metabolites and proteins and how they can be analysed using a variety of new technologies e.g. Deep Sequencing, proteomics, mass spectrometry, transcriptomics. Students will gain knowledge in applying, analysing and manipulating cells using current molecular methods and high-throughput technologies.Learning Outcomes
1. To manipulate bacterial cells using different cloning and transformation methods
2. To demonstrate knowledge and understanding of the fundamental principles and practices that underpin genomics, transcriptomics and proteomicsand their biotechnological applications.
3. To evaluate current research in -Omics and related biotechnological disciplines.
4. To acquire substantial quantities of information systematically and process it selectively and effectively.
5. To discriminate between the different approaches and techniques used in genomics, transcriptomics and proteomics
6. To develop ideas and opinions through the use of information from a wide variety of sources.
7. To communicate effectively knowledge of different -Omic technologies and their applications.
Conveners
- Dr Chiew Foan Chin